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. 2023 Mar 22;13:4682. doi: 10.1038/s41598-023-30899-z

Table 2.

Zinc masses (µg), concentrations (mg kg−1) and stable isotope compositions (δ66Zn) for total plants, and Zn stable isotope differences (Δ66Zn) and fractionation factors (ε66Zn).

Cacao clones (genotype) Total plant Isotopic differencesd Fractionation factors
Zn (µg) [Zn] (mg kg−1) δ66Zn (‰) 2SD (‰) Zn Uptakec (%) Δ66Zntotal-sold (‰) Δ66Znleaf-totald (‰) Δ66Znshoot-totald (‰) ε66Znuptakee (‰) ε66Znseq-mobf (‰)
B 5/7 [POU] 17 19  − 0.56 0.04 14.7  − 1.07  − 0.18  − 0.06  − 1.16 0.48
Catie 1000 15 27  − 0.40 0.03 12.8  − 0.91  − 0.27  − 0.06  − 0.98 0.49
CC 41a 21 23  − 0.46 0.04 18.7  − 0.98  − 0.19  − 0.04  − 1.09 0.58
CL 19/10 39 22  − 0.22 0.04 34.4  − 0.73  − 0.54  − 0.03  − 0.91 0.74
GU 207/Ha 18 29  − 0.33 0.03 15.3  − 0.84  − 0.10  − 0.05  − 0.92 0.32
GU 236/V 16 28  − 0.32 0.04 13.9  − 0.83  − 0.20  − 0.06  − 0.89 0.56
IMC 27 19 32  − 0.70 0.04 16.2  − 1.21  − 0.25  − 0.04  − 1.33 0.71
LP 1/41 [POU] 21 22  − 0.61 0.06 18.7  − 1.12  − 0.10 0.02  − 1.24 0.23
Matina 1–7b 45 21  − 0.63 0.05 39.4  − 1.15  − 0.09 0.00  − 1.49 0.18
NA 702 27 16  − 0.52 0.06 23.7  − 1.03  − 0.18  − 0.03  − 1.18 0.53
PNG 340 24 14  − 0.68 0.04 21.2  − 1.19  − 0.11  − 0.01  − 1.34 0.22
Pound 12/A [POU] 20 25  − 0.69 0.04 17.3  − 1.20  − 0.26  − 0.03  − 1.32 0.70
RB 46 14 18  − 0.64 0.05 12.5  − 1.15  − 0.47  − 0.07  − 1.23 0.78
RIM 179 42 22  − 0.61 0.04 36.8  − 1.12  − 0.11 0.01  − 1.42 0.32
SCA 9 27 26  − 0.45 0.06 23.7  − 0.96  − 0.19  − 0.06  − 1.10 0.50
SPA 9 [COL] 21 24  − 0.53 0.05 18.2  − 1.04  − 0.27  − 0.06  − 1.16 0.61
TARS 31 26 26  − 0.37 0.03 22.3  − 0.88  − 0.35  − 0.09  − 1.00 0.73
TSA 654 26 29  − 0.55 0.07 22.8  − 1.06  − 0.17  − 0.03  − 1.21 0.56
U 70 [PER] 20 50  − 0.27 0.07 17.2  − 0.78  − 0.16  − 0.05  − 0.86 0.67
Min 14 14  − 0.70 – 12.5  − 1.21  − 0.54  − 0.09  − 1.49 0.18
Max 45 50  − 0.22 – 39.4  − 0.73  − 0.09 0.02  − 0.86 0.78
Mean 24 25  − 0.50 – 21.0  − 1.01  − 0.22  − 0.04  − 1.15 0.52
1SD 8.8 7.4 – – 7.7 – – – – –
2SD – – 0.29 – – 0.15 0.24 0.06 0.36 0.36
NA 702 (0 µmol L−1 CdCl2)g 30 16  − 0.38 0.04 26.0  − 0.89  − 0.12 − 0.04  − 1.04 0.25
NA 702 (5 µmol L−1 CdCl2)g 26 15  − 0.39 0.05 22.8  − 0.90  − 0.03 − 0.02  − 1.03 0.09
NA 702 (20 µmol L−1 CdCl2)g 27 16  − 0.52 0.06 23.7  − 1.03  − 0.18 − 0.03  − 1.18 0.53
Mean 28 16  − 0.43 – 24.5  − 0.92  − 0.10 − 0.03  − 1.06 0.29
1SD 2 1 – – 3.12 – – – – –
2SD – – 0.12 – – 0.18 0.14 0.04 0.20 0.37

aMean values for the leaf, stem, and root aliquots of three separate CC 41 and GU 207/H plants (n = 3).

bMean value for the duplicate leaf, stem, and root aliquots of a single Matina 1–7 plant (n = 1).

cFraction of Zn (in %) taken up from the hydroponic solutions by the 4 cacao plants of the same genotype cultured in the same hydroponic container.

dIsotopic differences between total plants and the ZnSO4 added to the hydroponic solutions (Δ66Zntotal-sol), between leaves and total plants (Δ66Znleaf-total), and between shoots and total plants (Δ66Znshoot-total). SD represents the standard deviation. 2SD for individual samples represents the analytical precision of the Zn stable isotope data determined from multiple analyses of the Zn isotope standard that bracketed the sample runs.

eε66Znuptake denotes stable isotope fractionation factor for the uptake of Zn by the cacao plants from the hydroponic solutions.

fε66Znseq-mob represents the Zn stable isotope fractionation between the sequestered and mobile Zn in cacao plant.

gThe NA 702 genotypes treated with different CdCl2 concentrations had different samples sizes: 0 µmol L−1 CdCl2, n = 3; 5 µmol L−1 CdCl2, n = 2; 20 µmol L−1 Cl2, n = 1.