Skip to main content
. 2023 Mar 22;14:1589. doi: 10.1038/s41467-023-37266-6

Fig. 3. Intronic SNV in Trp53 associated with exon 8 skipping.

Fig. 3

A Schematic of a single nucleotide splice donor variant (yellow star; mm10, chr11:g.69589711T>G; c.1067+2 position of intron 8 of transcript NM_011640.3) within intron 8 of Trp53 (depicted as a series of boxes representing exons 7–11 with curved lines representing RNA splicing events). The variant appears to cause skipping of an exon (red curve). This result was found using the default splice variant window parameter (i2e3). B UMAP projection of single cells from MCB6C organoid-derived tumors with high confidence tumor cells (orange) and high confidence normal cells (blue) highlighted. C UMAP projection of single cells from MCB6C organoid-derived tumors overlaid with log2 expression values for Trp53. D Zoomed view of the UMAP projection showing cells containing the Trp53 exon skipping event (red dots). E Violin plots comparing the normalized junction score of the non-reference exon skipping event in cells with and without the Trp53 variant. Source data are provided as a Source Data file.