Fig. 6. Cell-based assay luciferase assay to monitor SARS-CoV-2 main protease activity of the wild-type (WT, open symbols) and catalytically inactive C145A mutant (black symbols) of Mpro: (A) dose response curve of the positive control GC376. (B) Dose response curve of compound 3b. The inhibition was assessed using the Rev-Nluc-based assay. The assay was performed as described in the ESI† Methods section. The inhibitor concentrations correspond to 3-fold serial dilutions from 125 to 0.02 μM. The RLUs are shown as the mean ± SD of three independent experiments performed in technical triplicate.
