Anti-SARS-CoV-2 activity on A549-ACE2 cells (RT-qPCR) and cell cytotoxicity of the 22 compounds, expressed as EC₅₀ in μM, the concentration at which 50% inhibition of the maximal signal is observed at 95% confidence interval.

Entry	R1	R2	Saturation on the LHS	RT-qPCR Beta EC50 (μM) [95% CI]	Cytotoxicity CC50 (μM) [95% CI]	Ratio cytotoxicity/RT-qPCR
3a′		—	Double bond	16 (N/A)	14 [2.8 to 73]	0.9
3a		H	Double bond	17 [13 to 23]	>50	>3
3b′		—	Double bond	13 [7.2 to 24]	6.6 [2.7 to 16]	0.5
3b		H	Double bond	3.7 [2.0 to 6.9] 3.5 [2.3to 5.6]a	12 [10 to 15]	3.3
					>50a
3c		H	Double bond	8.3 [6.3 to 11]	13 (N/A)	1.6
5	H	—	Saturated	>50	>50	N/A
6	Me	—	Saturated	>50	>50	N/A
7		—	Saturated	8.4 [5.5 to 13]	5.8 [3.0 to 11]	0.7
8a		H	Saturated	16 [5.2 to 51]	>50	>3
8b		H	Saturated	14 [8.5 to 22]	20 [8.2 to 48]	1.4
8c		H	Saturated	37 [23 to 59]	>50	1.4
8d′		—	Saturated	5.3 [4.0 to 7.1]	37 N/A	6.9
8d		H	Saturated	6.6 [4.3 to 10]	12 [6.7 to 21]	1.6
8e		H	Saturated	5.6 N/A	13 [7.6 to 21]	2.3
8f		H	Saturated	>50	>50	N/A
8h		H	Saturated	>17	>50	N/A
9a			Saturated	>40 [N/A]	>50 [N/A]	N/A
9b			Saturated	6.2 [N/A]	6.3 [3.7 to 11]	1.0
9c	Me		Saturated	20 [N/A]	17 [14 to 18]	0.8
9d			Saturated	>50	>50	N/A
9e			Saturated	1.4 [0.9 to 2.2] 4.4 [2.0 to 9.0]a	7.7 [5.2 to 12]	5.7
					>20a
10			Double bond	>50	—	N/A

^aAssays against cells infected with SARS-CoV-2 variant Omicron BA1.