Fig. 1.

Keap1 KD increases glucose uptake in neurons and astrocytes Glucose uptake rate measured as the rate of increase in 2-NBDG fluorescence intensity inside cells over time. Representative traces (A) and quantification (B) of the glucose uptake rate, normalized to WT neurons. WT n = 37; Nrf2-KO n = 24; Keap1-KD n = 41 neurons and WT = 18; Nrf2-KO n = 16, Keap1-KD n = 19 astrocytes. Representative traces (C) and quantification (D) of the glucose uptake rate after incubation in low glucose (1 mM) HBSS for 12 h. WT n = 13; Nrf2-KO n = 8, Keap1-KD n = 13 neurons and WT n = 6, Nrf2-KO n = 5, Keap1-KD n = 3 astrocytes. (E) Representative images of an experiment to measure 2-NBDG uptake rate in a neuron and an astrocyte, cell types outlined as indicated. 2-NBDG addition to the media leads to an increase in extracellular green fluorescence. Uptake of the fluorescent glucose leads to an increase in the fluorescent signal inside the cells. Scale bar: 20 μm. One-way ANOVA with Bonferroni post-hoc analysis for each group *p < 0.05, ***p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)