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. 2023 Mar 14;62:102672. doi: 10.1016/j.redox.2023.102672

Fig. 5.

Fig. 5

Inhibition of glycolysis doesn't redirect glucose utilization to pentose phosphate pathway(A) Scheme showing main pathways of cellular glucose metabolism and the role of iodoacetic acid (IAA) as an inhibitor of glycolysis (100 μM, 1 h, in the presence of 5 mM sodium pyruvate to maintain viability) and rotenone (rot) (5 μM, 20 min) as an inhibitor of complex I in the electron transport chain (ETC). (B-C) Colour-coded representative images of (D) NAD(P)H fluorescence lifetime (τ bound) and (G) percentage of enzyme-bound NAD(P)H (α bound) in basal conditions and after incubation with IAA or rotenone (D–G) Quantification in individual neurons and astrocytes of (D) NAD(P)H fluorescence lifetime (τ bound); (E) relative cytoplasmic enzyme-bound NADH levels; (F) relative cytoplasmic enzyme-bound NADPH levels and (G) percentage of enzyme-bound NAD(P)H (α bound) in basal conditions and after incubation with IAA or rotenone. Number of cells analysed is shown in brackets. Scale bar: 20 μm. Non-parametric Kruskal-Wallis ANOVA with post-hoc Dunn's test for each group, *p < 0.05, **p < 0.01, ***p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)