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. 2023 Mar 14;62:102672. doi: 10.1016/j.redox.2023.102672

Fig. 7.

Fig. 7

Nrf2 activation redirects glucose to NADH production when PPP is inhibited (A) Scheme showing main pathways of cellular glucose metabolism and the role of dehydroepiandrosterone (DHEA) as an inhibitor of pentose phosphate pathway (PPP) (100 μM, 24 h); and 2-AAPA (50 μM, 24 h) as an inhibitor of glutathione reductase (GR). (B-C) Colour-coded representative images of (D) NAD(P)H fluorescence lifetime (τ bound) and (G) percentage of enzyme-bound NAD(P)H (α bound) in basal conditions and after incubation with DHEA or 2-AAPA (D–G) Quantification in individual neurons and astrocytes of (D) NAD(P)H fluorescence lifetime (τ bound); (E) relative cytoplasmic enzyme-bound NADH levels; (F) relative cytoplasmic enzyme-bound NADPH levels and (G) percentage of enzyme-bound NAD(P)H (α bound) in basal conditions and after incubation with DHEA or 2-AAPA. Number of cells analysed is shown in brackets. Scale bar: 20 μm. Non-parametric Kruskal-Wallis ANOVA with post-hoc Dunn's test for each group, *p < 0.05, **p < 0.01, ***p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)