Table 2.
Primer Sequence.
| Gene | Primer Sequence (5’-3’) | Product size/bp |
|---|---|---|
| IL-1β | F:GCTTCATCTTCTACCGCCTGGAC | 159 |
| R:TTAGCTTGTAGGTGGCGATGTTGAC’ | ||
| IL-6 | F:TCTGGCAACGACGATAAGG | 154 |
| R:TGAAGTAAAGTCTCGGAGGATG | ||
| IFN-γ | F:ATACCCTTTCCAATGACT | 130 |
| R:GTCTCCACCAGTTTCTGT | ||
| TNF-α | F:AAATCTGCTGCTGGTCTT | 235 |
| R:CCATCATCGTCCTCACTA | ||
| ZO-1 | F: GGGGAAGACAACTGATGC | 159 |
| R: TTGTGATGTGCTGGGAGA | ||
| Claudin-1 | F: TGATGGTGGCTGCGATAC | 218 |
| R: AACAGGCGTGAAAGGGTC | ||
| Occludin | F: GCAGGATGTGGCAGAGGAATA | 119 |
| R: TGCGCTTGATGTGGAAGAGTT | ||
| β-actin | F:CCCCATTGAACACGGTATTGTC | 151 |
| R:GGCTACATACATGGCTGGGG |