Figure 10.

BcGRF8 broadly regulates genes related to N uptake, utilization, and signaling in Arabidopsis shoots (A) and roots (B). Briefly, 20-day-old seedlings in normal hydroponic conditions were transferred to N-free nutrient solution for 3 days and harvested for qRT-PCR after they were re-supplied with 3 mM ${\text{NO}}_3^{-}$ for 0, 1, 2, 4, or 8 h AtACTIN2 (At3g18780) was used as an internal control. AtGLN1.1, glutamine synthetase 1.1; GS2, glutamine synthetase 2; AtGLT1, glutamate synthetase 1; AtLBD38, lateral organ boundary domain 38; AtNIA2, ${\text{NO}}_3^{-}$ reductase 2; AtGDH2, glutamate dehydrogenase; AtGLN1.2, glutamine synthetase 1.2; AtNIA1, ${\text{NO}}_3^{-}$ reductase 1; AtNIR, ${\text{NO}}_2^{-}$ reductase. The values are means ± SDs of three replications. (*P < 0.05, **P < 0.01 vs. WT).