Figure 6.
BcGRF8 regulates BcNRT1.1 promoter activity. (A) GRF-related cis-acting elements in the BcNRT1.1 promoter regions. (B) Yeast one-hybrid assay. The auto-activation of promoters was tested on a synthetically defined medium lacking Ura in the presence of AbA. The interactions were determined on a synthetically defined medium lacking Leu in the presence of AbA (-Leu+AbA300). Negative control: AD-Empty+BcNRT1.1-1 promoter, AD-Empty+BcNRT1.1-2 promoter. (C) Reporter and effector gene constructs. (D) BcGRF8 activates two BcNRT1.1 promoter fragments. The experiments were repeated three times, and representative results from one experiment are shown. The error bars represent standard errors. (**P < 0.01, Student’s t-test).