Figure 3.

Interaction of XRCC3 with Rad51C but not with Rad51D and XRCC2. The cells were incubated for 2 h after 8 Gy γ-irradiation. (A) Immunoprecipitation of control HeLa and H158 (expressing HA-XRCC3) cell extracts with HA affinity matrix. The western blots were incubated with α-HA (top) or α-HsRad51 (bottom). HA-XRCC3 is detected in H158 cells but not in the untransfected control, and Rad51 co-precipitates with HA-XRCC3. (B) Immunoprecipitation analysis in H158 and HD19 (expressing HA-Rad51D) was done using HA affinity matrix. The western blots were incubated with: a, α-Rad51D; b, α-XRCC3; c, α-Rad51C; and d, α-XRCC2r.