Figure 5.

Lack of association of XRCC2 with Rad51. (A) Immunoprecipitation with HA affinity matrix in HD16 and HD19 cell extracts. The blots were incubated with α-HA (top) or α-HsRad51 (bottom). (B) Immunoprecipitation with Flag affinity matrix in wild type, irs1 and Flag-XRCC2 transformants of irs1 (IFX200). The blots were incubated with α-XRCC2r (top) or α-MmRad51 (bottom). (C) Immunofluorescence detection of GFP-XRCC2 in stable transformants of wild-type V79 cells 2 h after 0 or 8 Gy γ-irradiation (top). The same cells stained with DAPI (bottom).