Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Mar 3;32:64–79. doi: 10.1016/j.omtn.2023.02.032

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Efficient correction of STGD1-related variants by CRISPR-Cas9 in hiPSCs

(A) FRIMO003-A hiPSC clones were subjected to Sanger sequencing to analyze gene editing in the locus specified after CRISPR-Cas9. Representative captures of chromatograms from Sanger results of parental and one edited clone are shown. (B) Pie chart of the percentage of edited clones in FRIMOi003-A cells. (C) As in (A) but with FRIMOi004-A. Representative capture of edited clones with PAM modification is also shown. (D) Pie chart of the percentage of edited clones according to variant correction and PAM modification in FRIMOi004-A cells. (E) Summary of the on-target events in total screened clones for each hiPSC line after CRISPR-Cas9-mediated gene editing. (F) Pie chart of the percentage of edited clones in FRIMOi004-A according to variant correction and PAM modification after cell culture conditions optimization. (G) Comparison between CRISPR-Cas9-mediated gene editing efficiency assays after normal and optimized conditions in the FRIMOi004-A hiPSC line.