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Correction of the ABCA4 variants does not result in off-target events after CRISPR-Cas9-mediated gene editing
(A) Representative captures of chromatograms showing Sanger sequencing reads of PCR products from predicted off-targets for sgRNA2. On top are reference and consensus sequences and on the bottom the sequence of one edited clone. The sequence homologous to sgRNA2 and the adjacent PAM motif are highlighted (in red). (B) As in (A) but for sgRNA6.
