Table 3.

Sequencing results from selected sgRNA2 and sgRNA6 off-targets

sgRNA	Off-target ID	DNA sequencea,b	Chr	Position	Strand	Mismatches	Gene	Location	Del/ins/indel	Single-nt mod.
sgRNA2	OT-1	TTCTTCAGtTGTaCtGACTCTGG	chr12	80,295,911	−	3	OTOGL	intron 31	0/2	0/2
	OT-2	TcaTTCAGGTGTGaGGACTCTGG	chr22	46,361,593	+	3	CELSR1	exon 35	0/2	0/2
sgRNA6	OT-3	GCATcCAGAGAAAGCTaTGTAGG	chr1	112,903,490	−	2	–	intergenic	0/33	0/33
	OT-4	cCATGCAGAGAAAGCTtTGaAGG	chr8	79,763,025	+	3	HEY1	exon 5	0/33	0/33
	OT-5	GCATGCAGAGgAgGCTtTGTAGG	chr1	170,663,322	−	3	PRRX1	exon 1	0/33	0/33
	OT-6	GaATGCAGAGAAgGCTtTGTGGG	chr1	183,127,284	+	3	LAMC1	exon 17	0/33	0/33
	OT-7	aCATGCAGtGAAAGCTGTGgAGG	chr6	72,272,584	−	3	RIMS1	intron 22	0/33	0/33

^aNucleotides with mismatch compared with the sgRNA reference sequence are in lowercase.

^bPAM sequence is in italic.