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. 2022 Dec 22;16(4):784–798. doi: 10.1111/1751-7915.14195

FIGURE 3.

FIGURE 3

pNZ8048 delivery to L. lactis MG1363 GFP+ from B. subtilis. (A) The average values of pNZ8048 transfer efficiency for B. subtilis to L. lactis matings. B. subtilis 168 and PY79 harbouring pNZ8048 were designed as donor strains and MG1363 with chromosomally integrated and constitutively expressed sfGFP as a recipient strain. The statistical analysis was performed in Prism (t‐test, ns: p = 0.2961), the error bars show the standard deviation (SD), n = 4. (B) Colony formation after replica plating on GM17 selective medium with reduced concentrations of chloramphenicol. The yellow arrows indicate B. subtilis erythromycin resistant colonies. The yellow circles indicate colonies of L. lactis transformants picked for further analysis. (C) Micrographs of L. lactis MG1363 GFP+ transformants. (D) Gel electrophoresis of isolated plasmids from L. lactis transformants, the pNZ8048 lane corresponds to the plasmid isolated from L. lactis MG 1363 pNZ8048 strain. (E) PCR analysis of transformant 9. The yellow markers indicate the band representing chloramphenicol cassette.