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Effect of G4 loop length on the binding affinity of RGGF. The EMSA was performed using RGGF (lanes 2, 4, 6, and 8) with 32P-labeled Htelo (lanes 1 and 2), dL131dG4 (lanes 3 and 4), dL121dG4 (lanes 5 and 6), or dL111dG4 (lanes 7 and 8). The DNA–protein complexes were resolved by 6% polyacrylamide gel electrophoresis and visualized by autoradiography.
