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Binding activity of RGGF to G4 BCL-2. The equilibrium binding curve was obtained by calculating the fraction of 32P-labeled BCL-2 at varying RGGF concentrations. The dissociation constant (Kd) was ascertained by fitting the data to the appropriate equation. The DNA–protein complexes were resolved by 6% polyacrylamide gel electrophoresis and visualized by autoradiography.
