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. Author manuscript; available in PMC: 2023 Mar 23.
Published in final edited form as: Gastroenterology. 2021 Sep 25;162(1):209–222. doi: 10.1053/j.gastro.2021.09.044

Figure 4.

Figure 4.

SOX9 knockdown promotes intestinal differentiation in organoid models of CRC. (A) mRNA expression heat map of intestinal differentiation (blue) and stem cell (red) markers in control and indicated SOX9 shRNA human colon organoids. (B) Immunoblot (Sox9 and vinculin) of mouse control or indicated Sox9 shRNA ApcKO KrasG12D colon organoids. Proliferation by CellTiterGlo; mean ± SD; Student’s t test: ***P < 0.005, ****P < 0.001. (C) Ki67 IHC quantification of indicated fixed mouse ApcKO KrasG12D colon organoids; mean ± SD; Student;s t test ***P < 0.005, ****P < 0.001. (D) Sox9, Lgr5, Lrig1, Prom1, Axin2, Ascl2, Krt20 mRNA expression in ApcKO KrasG12D colon organoids according to qRT-PCR; mean ± SD; Student’s t test: **P < 0.01, ***P < 0.005, ****P < 0.001. (E) ApcKO KrasG12D Sox9 xenograft schematic. Primary tumor xenograft growth curve and day 30 quantification. Representative images of xenograft tumors. (F) Immunoblot (Sox9 and vinculin) of xenograft tumors. (G) Ki67 and Muc2 immunohistochemistry and Alcian blue–periodic acid Schiff (AB-PAS) staining of xenograft tumors; scale bars = 100 μm and 20 μm. Abbreviations as in Figures 1 and 2.