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. Author manuscript; available in PMC: 2023 Mar 23.
Published in final edited form as: Gastroenterology. 2021 Sep 25;162(1):209–222. doi: 10.1053/j.gastro.2021.09.044

Figure 6.

Figure 6.

SOX9 directly activates PROM1 via a Wnt-responsive intronic enhancer. (A) Volcano plot displaying log2 fold change of differentially expressed genes (SOX/GFP) along x-axis (FDR <0.05). Blue points mark genes significantly associated with SOX9-binding or differential H3K27ac. (B) Prom1 mRNA expression in indicated ApcKO KrasG12D colon organoids according to qRT-PCR; mean ± SD; Student’s t test: ****P < 0.001. (C) PROM1 mRNA expression in human CRC with WT, Het, or homozygous (Homo) SOX9 mutations. TCGA, The Cancer Genome Atlas. (D) mRNA and protein expression of PROM1 in indicated cell lines ± 0.5 μg/mL Dox. (E) IGV gene track of PROM1 intron1; signal tracks of H3K27ac (red) and V5-ChIP (blue) in indicated conditions; peak scale displayed in top right. (F) PROM1 enhancer reporter assay: phase-contrast and fluorescence images and quantification of HEK293T transiently transfected with indicated plasmids and/or treated with WNT3A. Abbreviations as in Figures 1, 2, and 5.