Fig. 10.

Silencing of circHMCU receded tumour growth in vivo. MDA-MB-231 cells were transfected with sh-NC or sh-circHMCU. A Tumor volume was measured every week. B Tumor weight was examined after mice were sacrificed. C The levels of circHMCU, miR-4458 and PGK1 were measured by qRT-PCR. D The protein level of PGK1 was assessed by western blot. E The levels of ki-67 and PGK1 were detected by IHC. *P < 0.05