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. 2023 Mar 23;160:12. doi: 10.1186/s41065-023-00275-y

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — MiR-4458 directly target PGK1. A The predicted binding site between miR-4458 and PGK1was exhibited. B The binding relationship between miR-4458 and PGK1 was verified by dual-luciferase reporter assay. C The expression of PGK1 in normal tissues (n = 66) and tumor tissues (n = 66) was tested by qRT-PCR. D The expression of PGK1 protein in in normal tissues and tumor tissues was tested by western blot. E PGK1 protein expression was measured in BC cell lines (MDA-MB-231 and MCF-7) and human normal mammary epithelial cell (MCF10A). F Pearson analysis was conducted to analyze the correlation between miR-4458 and PGK1 in BC tissues. G The expression of PGK1 protein was exhibited by western blot.*P < 0.05