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Primers sequences used for PCR
| Name | Primers for PCR (5’-3’) | |
|---|---|---|
| hsa_circ_0000247 | Forward | CTGTTCACGCAGGGGAAACT |
| Reverse | AGCAGCTAAGATGTCACTGGC | |
| PGK1 | Forward | CCACTGTGGCTTCTGGCATA |
| Reverse | ATGAGAGCTTTGGTTCCCCG | |
| HMCU | Forward | CCACTGGGCCTCTCTAAACA |
| Reverse | CTCCCACCCTGATTCCAAGT | |
| miR-4458 | Forward | TCGGCAGGAGAGGTAGGTGTGGAAGAA |
| Reverse | CTCAACTGGTGTCGTGGAG | |
| β-actin | Forward | CTTCGCGGGCGACGAT |
| Reverse | CCACATAGGAATCCTTCTGACC | |
| U6 | Forward | CTCGCTTCGGCAGCACA |
| Reverse | AACGCTTCACGAATTTGCGT |
