Extended Data Fig. 4 |. Direct binding of IL2RA regulators at the IL2RA locus.

Chromatin accessibility measured by ATAC-Seq in AAVS1 control knockouts is shown in black. ATAC-Seq data are shown as normalized read coverage; samples were normalized using the size factors from DESeq2. Results from previous CRISPR activation (CRISPRa) screen³⁸ tiling the IL2RA locus in Jurkat cells is shown in pink. CRISPRa tracks show the log₂ enrichment of guide RNAs in cells expressing high, mid, or low levels of IL2RA compared to background. Public ChIP-Seq data for IL2RA regulators in various subsets of human CD4 + T cells (STAT5A, STAT5B, ETS1, GATA3, MYB) or engineered bulk T cells (IRF4) are shown in green. ChIP-Seq data are shown as background subtracted binding in reads per million. ATAC-Seq peaks that were significantly differentially accessible in each knockout are shown in blue. The location of a matching binding motif in a significantly differentially accessible peak for each transcription factor is shown in orange. Where available, public ChIP-Seq tracks are from either two independent studies or individual donors: ETS1^48,81, GATA3⁴⁷, IRF4⁸², MYB⁸³, STAT5A and STAT5B⁸⁴. chr, chromosome.