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. 2022 Nov 10;2(11):1404–1417. doi: 10.1158/2767-9764.CRC-22-0266

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© 2022 The Authors; Published by the American Association for Cancer Research

This open access article is distributed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license.

PMC Copyright notice

M2 macrophages are highly responsive to ZL-1201 and CD47 blockade. A,CD80, CD86, CXCL10, CD163, and CD206 transcript levels in monocytes and differentiated M0, M1, and M2 macrophages. B, Flow cytometric histogram photos showing CD80 and CD206 surface protein levels in differentiated M0, M1, and M2 macrophages. C, MCP1, CXCL10, and IL10 secretion by differentiated M0, M1, and M2 macrophages. In vitro phagocytosis using HCC1954 (D), FaDu (E), Toledo (F), and Raji (G) cancer cells and differentiated M0, M1, or M2 macrophages under the treatment conditions as shown. Fold phagocytosis was calculated relative to nontreated (NT) group and shown as mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001.