FIGURE 4.

Depletion of tumor cell–autonomous SHP2 relieves immune suppression. A, Plots comparing the ratio of the frequency of CD4⁺ and CD8⁺ as CD4/CD8 in isolated spleen (left) and lung tissues (right) of each group. B, Quantification of TIM3⁺LAG3⁺ population as a frequency of CD45⁺CD4⁺ cells in isolated spleens of each group. C, Quantification of TIM3⁺LAG3⁺ population as a frequency of CD45⁺CD4⁺PD-1⁺ cells in isolated lung tissues of each group. D, Quantification of TIM3⁺ population as a frequency of CD45⁺CD8⁺ cells in isolated spleens (left) and lung tissues (right) of each group. E, Quantification of TIM3⁺LAG3⁺ population as a frequency of CD45⁺CD8⁺ cells in isolated spleens of each group. F, Quantification of TIM3⁺LAG3⁺ population as a frequency of CD45⁺CD8⁺PD-1⁺ cells in isolated lung tissues of each group. G, Quantification of F4/80⁺ population as a frequency of CD45⁺CD11b⁺ cells in isolated lung tissues of each group. H, Plots comparing the ratio of CD86⁺ and CD206⁺ in F4/80⁺ population as M1/M2 in isolated lung tissues of each group. In all panels. NS, no significance. *, P < 0.05; **, P < 0.01; ***, P < 0.001; n = 4 for shScramble and shPTPN11 146, n = 5 for shPTPN11 369 in pulmonary tumor panels, n = 4 for each group in spleen panels.