SHP2 regulates the expression of MHC class I via a balance between MAPK and STAT1 signaling in MBC cells. A, Histogram of cell surface analysis of H-2 in D2.A1 cells treated with different growth factors, mouse IFNγ (200 ng/mL) and TNO155 (5 μmol/L). B, Bar graph comparing fold change of H-2 MFIs induced by different growth factors, IFNγ and TNO155 compared with DMSO+ IFNγ. NS, not significant; **, P < 0.01; ***, P < 0.001; n = 3. C, Immunoblotting showing differential STAT1 and ERK1/2 phosphorylation in D2.A1 cells treated with different growth factors, IFNγ, and TNO155. D, Bar graph comparing fold change of H-2 MFI induced by different growth factors and IFNγ compared with IFNγ alone with different inhibitors in D2.A1 cells. NS, not significant; **, P < 0.01; ***, P < 0.001; n = 3.