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. 2023 Jan 25;3(1):130–139. doi: 10.1158/2767-9764.CRC-22-0420

FIGURE 2.

FIGURE 2

Chromogenic multiplex expression analysis of different biomarkers in formalin-fixed paraffin-embedded tissue of a patient's tumor with a survival of 13 months (34364) and another 3.5 months (34368) after treatment and quantitative analysis using Halo image analysis platform. A, Representative field for chromogenic multiplex for D45(brown)/Ki67(purple)/CD3(teal)/CD163(yellow) for case 34364 (left) and 34368 (right) tissues. Color deconvolution for each marker was obtained using HALO image analysis, and quantitative analysis for Ki67 and CD163 is shown in E (CD45 and CD3 color deconvolution and HALO quantification is shown in Supplementary Fig. S2). B, Representative field for chromogenic multiplex for PD- L1 (brown)/CD8 (purple) with their respective deconvolution images and quantification on E. C, Representative field for CD31 IHC in brown with image deconvolution with quantitative analysis in E. D, Representative field for CD68 IHC in yellow with image deconvolution with quantitative analysis in E.