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Effect of prenatal MOUD and Alcohol use on human placental mRNA expression. The equation C=2^Tnormalizer/2^Ttarget was used to quantify mRNA levels normalized to the housekeeping gene 18s. Raw data, prior to logit transformation, are presented as mean ± SD. Preliminary ANOVA analyses yielded significant main effects of group on NLRP3 and SERT mRNA despite appearance of outliers.
