Figure 4.

Activation of the glycolytic system. Diagram of energy metabolism pathways and mass spectrometry before and 2 d (48 h) after axotomy. MPC transports pyruvate, the end-product of the glycolytic system, for metabolism in the TCA cycle. Mass spectrometry was performed for the metabolites of glycolysis and the TCA cycle, the end-product ATP, and the coenzyme NAD⁺. Glycolytic intermediates, NAD⁺ and ATP were significantly decreased after axotomy. These results are shown in a boxplot containing the interquartile range and centered bars as the median (n = 5 mice per group). All examinations were performed continuously 7 mm from the sectional end of the right sciatic nerve with transection and the corresponding lesion on the left side. *p < 0.05, **p < 0.01, Mann–Whitney U test. CS, citrate synthase; F6P, fructose 6-phosphate; G6P, glucose-6-phosphate; GA3P, glyceraldehyde-3-phosphate; HK, hexokinase; IDH, isocitrate dehydrogenase; LDH, lactate dehydrogenase; NAD⁺, nicotinamide adenine dinucleotide; PFK, phosphofructokinase; PDH, pyruvate dehydrogenase; TCA cycle, tricarboxylic acid cycle.