Figure 8.

TP protected HG-treated MPC5 cells by activating Nrf2 to scavenge ROS and inhibit the NLRP3 inflammasome pathway. (A) The protein levels of Nrf2 and HO-1 in MPC5 cells were detected by WB; (B) MPC5 cell activity was detected by MTT; (C–E) The levels of MDA, SOD, and GSH in MPC5 cells were detected; (F) ROS levels in MPC5 cells were detected by a DCFH-DA fluorescence probe; (G) WB was used to detect the levels of NLRP3 inflammasome pathway-related proteins; (H) The secretion levels of inflammatory cytokines IL-1β and IL-18 were detected by ELISA; Data were expressed as mean ± SD, and the cell experiments were independently repeated three times. Comparison between two groups was performed by the t-test. *p < 0.05, **p < 0.01, ***p < 0.001.