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. 2023 Mar 23;12:e83979. doi: 10.7554/eLife.83979

Key resources table.

Reagent type (species) or resource Designation Source or reference Identifiers Additional information
Genetic reagent (D. melanogaster) w[1118]CS Other Gift from A Simon
Genetic reagent (D. melanogaster) UAST-sxc(Ogt)RNAiCLb38 PMID:24706800 Gift from C Lehner
Genetic reagent (D. melanogaster) Gr5a-GAL4 Bloomington Drosophila Stock Center BDSC: 57592
Genetic reagent (D. melanogaster) Tubulin-GAL80ts Bloomington Drosophila Stock Center BDSC: 7018
Genetic reagent (D. melanogaster) UAS-Pcl FlyORF FlyORF: F001897
Genetic reagent (D. melanogaster) Pclc429 Other Gift from N Liu
Genetic reagent (D. melanogaster) UAS-Rpl3-3XFLAG PMID:29194454 Gift from D Dickman
Genetic reagent (D. melanogaster) UAS-LT3-Dam Other Gift from AH Brand
Genetic reagent (D. melanogaster) UAS-LT3-Dam::Pcl PMID:33177090
Genetic reagent (D. melanogaster) UAS-LT3-Dam::OGT This paper See the Materials and methods
Genetic reagent (D. melanogaster) Gr64f-GAL4 Bloomington Drosophila Stock Center BDSC: 57669
Genetic reagent (D. melanogaster) UAS-Sr Bloomington Drosophila Stock Center BDSC: 26553
Genetic reagent (D. melanogaster) UAS-rlWT Bloomington Drosophila Stock Center BDSC: 36270
Genetic reagent (D. melanogaster) UAS-rlSem Bloomington Drosophila Stock Center BDSC: 59006
Genetic reagent (D. melanogaster) rl1 Bloomington Drosophila Stock Center BDSC: 386
Genetic reagent (D. melanogaster) rl RNAi Bloomington Drosophila Stock Center BDSC: 34855
Genetic reagent (D. melanogaster) OGA RNAi Bloomington Drosophila Stock Center BDSC: 41882
Genetic reagent (D. melanogaster) OGT1 PMID:26348912 Gift from D van Aalten
Genetic reagent (D. melanogaster) OGTK872M PMID:26348912 Gift from D van Aalten
Antibody Mouse monoclonal anti-Flag Sigma Cat#: F1804, RRID: AB_262044 3:50
Peptide, recombinant protein Dynabeads Protein G Thermo Fisher Scientific Cat#: 10004D
Peptide, recombinant protein T4 DNA ligase New England Biolabs Cat#: M0202S
Commercial assay or kit NEBuilder HiFi DNA Assembly kit New England Biolabs Cat#: E5520S
Commercial assay or kit ThruPLEX Kit Takara Cat#: 022818
Chemical compound, drug OSMI-1 Sigma Cat#: SML1621
Chemical compound, drug EED226 Axon Medchem Cat#: 2701
Chemical compound, drug Trametinib LC labs Cat#: T-8123
Chemical compound, drug TRIzol LS Reagent Thermo Fisher Scientific Cat#: 10296010
Software, algorithm Autospike3.9 Syntech http://www.ockenfels-syntech.com/products/signal-acquisition-systems-2/
Software, algorithm Prism 9 GraphPad RRID: SCR_002798
Software, algorithm Python Python RRID: SCR_008394