Table 2.
Primers used in this study
| Sr. No | Gene | Primer sequence (5´–3´) | Anneal. temperature | Product size (bp) | References |
|---|---|---|---|---|---|
| 1- | uidA |
F:ATCACCGTGGTGACGCATGTCGC R:CACCACGATGCCATGTTCATCTGC |
50 | 486 | Bashir et al. (2012) |
| 2- |
bla SHV-1 |
F: GGCCGCGTAGGCATGATAGA R: CCCGGCGATTTGCTGATTTC |
60 | 714 | Wajid et al. (2019) |
| 3- | bla TEM-1 |
F: CAGCGGTAAGATCCT TGAGA R: ACTCGCCGTCGTGTAGATAA |
55 | 643 | Wajid et al. (2019) |
| 4- | bla CTX-M1 |
F: AACCGTCACGCTGTTGTTAG R: TTGAGGCTGGGTGAAGTAAG |
55 | 766 | Wajid et al. (2019) |
| 5- | bla CTX-M15 |
F: CAATGTGCAGCACCAGTAA R: CGCAATATCATTGGTGGTG |
58 | 540 | Wajid et al. (2019) |
| 6- | bla CMY-2 |
F: TGGCCGTTGCCGTTATCTAC R: CCCGTTTTATGCACCCATGA |
55 | 870 | Wajid et al. (2019) |
| 7- | bla SHV-12 |
F:GGTTATTCTTATTTGTCGCTTCTT R:TACGTTACGCCACCTGGCTA |
55 | 1233 | Wajid et al. (2019) |
| 8- | OXA-1 |
F: AATGGCACCAGATTCAACTT R: CTTGGCTTTTATGCTTGATG |
55 | 640 | Li & Wang (2017) |
| 9- | bla KPC |
F:CGTCTAGTTCTGCTGTCTTG R:CTTGTCATCCTTGTTAGGCG |
55 | 538 | Farajzadeh- Sheikh et al. (2020) |
| 10- | bla VIM |
F:GTTTGGTCGCATATCGCAAC R:AATGCGCAGCACCAGGATAG |
60 | 389 | Awan et al. (2021) |
| 11 | bla GES |
F: GTTTTGCAATGTGCTCAACG R: TGCCATAGCAATAGGCGTAG |
56.65 | 371 | Poirel et al. (2011) |