Fig. 1. Sequence and conformational properties of FtsQ1-99.

a The amino-acid sequence. The helical region is underlined, with Ala residues therein in green; acidic and basic residues are shown as red and blue, respectively, throughout the sequence. b ¹H-¹⁵N HSQC spectrum of uniformly ¹³C-¹⁵N-labeled FtsQ1-99 at 370 μM, acquired at 800 MHz and 305 K in 20 mM Tris (pH 6.85) with 50 mM of NaCl. c Helical wheel representation for core helical residues 48–72. Acidic, basic, Ala, and other residues are shown in red, blue, green, and grey, respectively. Arg49 and Arg50 define a 100° arc, in which Ala53, Ala57, Ala60, Ala64, and Ala71 fall.