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. 2023 Mar 23;13:4788. doi: 10.1038/s41598-023-30870-y

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Iodine k-edge subtraction imaging (KES). (a) Shows a representative cross-section through a scan (33 keV) of a pig lung injected with different concentrations of iodine/agarose gel mixes. The white arrows point to vessels filled with the contrast agent. Differences in iodine concentration can not be observed. (b) CT scan of a phantom with known iodine concentrations (overlaid in pseudo-color), from which an iodine concentration calibration function was retrieved (color bar right). (c) The Subtraction image of two scans at 33.5 and 33 keV demonstrates a high specificity for iodine, which in turn allows to quantify difference in the iodine concentration. (d) Quantification of the iodine concentrations (color coded) using the calibration function (b).