Figure 4.

(a) Vesicle containing cholesterol and a heterogeneous vesicle containing short-chain lipids. Relationships between membrane properties (fluorescence anisotropy and polarity) and liposome size depending on (b) cholesterol content (flow rates of the ethanol and buffer phases fixed at 100 and 1900 µL h⁻¹, respectively) and (c) 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC) content (flow rates of the ethanol and buffer phases fixed at 400 and 1600 µL h⁻¹, respectively). (d) The membrane properties and size depending on DHPC contents when the ethanol content was reduced (flow rates of the ethanol and buffer phases fixed at 100 and 1900 µL h⁻¹, respectively).