Figure 2.

Enhanced neuron transduction by i.v. injected BR1N

(A) Immunohistochemistry of brain sagittal sections from male mice treated i.v. with BR1 or BR1N. The sagittal sections were double-immunolabeled for CD31, a microvasculature endothelial cell marker, and NeuN, a neuronal marker. Scale bar, 100 μm. (B) Graphs showing the number of cells double-positive for GFP and CD31 (transduced endothelial cells, left graph) and those double-positive for GFP and NeuN (transduced neurons, right graph). Asterisks show statistically significant differences (n = 4 mice per group; ∗∗∗p < 0.001 and ∗∗∗∗p < 0.0001 by unpaired t test). (C) Percent ratio of transduced cell types to total brain parenchymal cells (GFP-positive and CD31-negative cells) (n = 4 mice). Cerebral sections were immunolabeled for CD31, NeuN, the astrocyte marker S100, or the microglia marker Iba1. All error bars show SEM.