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. 2023 Mar 23;14:1617. doi: 10.1038/s41467-023-37427-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a, b Dorsal view of normal and affected anterior orbital (ao) showing a meandering shape mostly observed on the offspring from MTX treated males. c Quantification of the percentage of embryos presenting affected or normal anterior orbital cartilages. Numbers in the graph represent the analyzed embryos. d Lateral view of larvae presenting normal (trowel shape), mild (bended shape), and strong (hook shape) deformities of the basihyal cartilage. e Quantification of the percentage of embryos presenting normal (non-affected), mild, or strong basihyal cartilage abnormalities observed on the offspring from MTX-treated males. Numbers in the graph represent the analyzed embryos. Statistical analyses were performed using a contingency table followed by two-sided Fisher’s exact test and each treatment were compared with the control independently. *P = 0.0329, **P = 0.0059, ***P = 0.0006.