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. 2023 Mar 23;14:1617. doi: 10.1038/s41467-023-37427-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Histogram representing the comparison from sperm sncRNA on control (Ctrl1-3) and 10MTX (MTX-1-3) treated males. b Histogram displaying biotypes of tRNAs and rRNAs from sperm of MTX treated males. See also Supplementary Data 1 for a and b. c Volcano plot of depicting the fold changes in sperm sncRNAs identified as being differentially expressed within control versus MTX-treated males. Analysis of differential expression was performed by using DESeq2 which use the two-tailed Wald test. d MA plot displaying normalized counts (base mean) for different sncRNAs. Dotted lines depict thresholds values for significantly up and down-regulated (±≥1 log₂ fold change and -log₁₀ P value ≥ 1.3). See also Supplementary Data 2 for c and d.