Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Mar 23;14:1617. doi: 10.1038/s41467-023-37427-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — Histogram comparing sperm RNA methylations on control and MTX analyzed by UHPLC-MS-MS in 50–90 nt (a) and 20–50 nt (b) fractions. Data represent two biologically independent replicates (n = 2) composed by RNA isolated from 9 males’ sperm. Statistical analysis was performed by two-tailed unpaired t-student’ test. Values are mean ± S.D. (c) Schematic representation of modified nucleotides in the tRNA at secondary and tertiary structure. d RT-qPCR for methyltransferases of m1A (TRMT6) and m5C (DNMT2 and NSUM2) on testis from control and MTX treated males, gene expression was normalized using Rpl7 and Ef1 as housekeeping genes. Statistical analysis was performed by using the two-tailed unpaired t-student’ test. Each dot represents a biologically independent sample composed by RNA isolated from individual male testis. Valures are means ± SEM.