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. 2023 Mar 23;13:4806. doi: 10.1038/s41598-023-31964-3

Table 2.

Effect of Cut (1–5 where 1,3 and 5 is the first, second and third cut in the three-cut system, and 2 and 4 is the first and second cut in the two-cut system) and crop (T, Timothy; T + RC, Timothy + red clover; RG, perennial ryegrass) on silage feed quality parameters averaged across DM and additive treatments (n = 4).

Cut Three cuts Two cuts SEM P value
T T + RC RG T T + RC RG Cut Crop Cut × crop C1 C2 C3 C4 C5
DM, g/kg 1st 31.1 31.9 29.3 35.2 37.3 34.8 4.666 0.481 0.918 0.999 0.200 0.198 0.916 0.724 0.994
2nd 34.3 36.2 33.1 35.3 32.7 34.4
3rd 31.0 28.8 29.9
Organic matter, g/kg DM 1st 930 932 924 942 945 932 0.762 < 0.001 < 0.001 < 0.001 < 0.001 < 0.001 < 0.001 < 0.001 < 0.001
2nd 930 923 929 940 930 918
3rd 931 911 911
NDFom, g/kg DM 1st 508 501 425 576 586 543 8.958 < 0.001 < 0.001 < 0.001 < 0.001 < 0.001 < 0.001 < 0.001 < 0.001
2nd 487 429 487 562 515 535
3rd 449 370 400
iNDF, g/kg NDFom 1st 119 112 121 230 220 246 6.268 < 0.001 < 0.001 < 0.001 < 0.001 < 0.001 0.011 < 0.001 0.518
2nd 155 159 254 204 200 205
3rd 143 174 121
OMD, % 1st 75.3 75.9 77.3 65.8 66.2 66.1 0.464 < 0.001 < 0.001 < 0.001 < 0.001 < 0.001 < 0.001 0.859 < 0.001
2nd 73.7 75.3 67.9 68.1 70.1 69.0
3rd 75.5 76.3 77.9
CP, g/kg DM 1st 147 126 121 103 89.3 95.4 1.709 < 0.001 < 0.001 < 0.001 < 0.001 < 0.001 < 0.001 < 0.001 0.008
2nd 141 144 124 106 109 113
3rd 113 158 112
sCP, g/kg CP 1st 790 755 781 580 557 721 24.85 < 0.001 < 0.001 0.012 0.002 < 0.001 0.181 < 0.001 0.002
2nd 582 538 704 596 486 660
3rd 510 459 664
WSC, g/kg DM 1st 37.6 46.4 91.5 60.4 58.0 83.8 21.78 0.639 0.160 0.641 0.961 0.619 0.649 0.218 0.502
2nd 41.8 56.0 64.8 56.9 51.8 78.3
3rd 113 50.8 77.1

DM, dry matter; NDFom, neutral detergent fibre; iNDF, indigestible NDF; OMD, organic matter digestibility; CP, crude protein; sCP, soluble crude protein; WSC, water-soluble carbohydrates. C1 is the contrast three versus two cuts per season overall; C2 is the contrast three versus two cuts per season, 1st cut; C3 is the contrast three versus two cuts per season, 2nd cut; C4 is the contrast T versus RG; C5 is contrast T versus T + RC.