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. 2023 Mar 23;13:4806. doi: 10.1038/s41598-023-31964-3

Table 5.

Effect of wilting rate (target 22.5% or 37.5% DM) and silage additive (without or with) on silage fermentation characteristics averaged across cuts and crop types (n = 15).

22.5% DM 37.5% DM SEM P value
Without With Without With Cut DM Cut × DM Add Cut × Add DM × Add Cut × DM × Add
Lactic acid, g/kg DM 61.7 31.4 26.9 13.9 2.475 < 0.001 < 0.001 0.183 < 0.001 0.003 0.001 0.025
Acetic acid, g/kg DM 17.7 7.83 8.70 3.41 0.861 < 0.001 < 0.001 0.493 < 0.001 0.022 0.012 0.290
Propionic acid, g/kg DM 1.54 0.98 0.99 0.91 0.079 < 0.001 < 0.001 0.619 < 0.001 0.094 0.005 0.637
Butyric acid, g/kg DM 8.50 5.64 3.95 1.71 0.705 < 0.01 < 0.001 0.345 < 0.001 0.140 0.662 0.534
Ethanol, g/kg DM 3.44 2.61 2.70 1.36 0.254 < 0.001 < 0.001 0.433 < 0.001 0.003 0.319 0.720
Formic acid, g/kg DM 0.70 12.3 0.28 5.22 0.377 < 0.001 < 0.001 0.505 < 0.001 < 0.001 < 0.001 0.821
pH 4.17 4.14 4.51 4.73 0.050 < 0.001 < 0.001 < 0.001 0.050 0.312 0.021 0.002
NH3, g/kg total N 63.4 41.3 38.0 28.8 2.102 < 0.001 < 0.001 0.020 < 0.001 0.877 0.004 0.854

Cuts are numbered 1, 3 and 5 for the three consecutive cuts in the three-cut system and 2 and 4 for 1st and 2nd cut in the two-cut system, respectively. Cut × DM: Interaction between cut and dry matter; Add, Silage additive (with or without), Cut × Add: Interaction between cut and Add, DM × Add: interaction between DM and Add, Cut × DM × Add, three-way interaction between Cut, DM and Add.