Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Mar 23;14:1612. doi: 10.1038/s41467-023-37056-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Crown 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a Retention assay using protoplasts showing the secretion index (ratio extra/intracellular Amy-HDEL activity) with cargo alone (either Amy-HDEL or Amy-KDEL) or with co-expressed A. thaliana ERD2b (At) and 12 further ERD2 orthologs from the eukaryotes Ostreococcus lucimarinus (Oi), Acanthamoeba castellanii (Ac), Phytophthora infestans (Pi), Chondrus crispus (Cc), Galdieria sulphuraria (Gs), Homo sapiens (HsERD2), Hypsibius dujardini (Hd), Thalassiosira pseudonana (Tp), Puccinia graminis (Pg), Kluyveromyces lactis (KlERD2), Trypanosoma brucei (Tb) and Saccharomyces cerevisiae (Sc). Transfection efficiencies were normalised by the internal marker GUS established at 5 standard OD units as described in materials and methods. Percentages in brackets refer to the sequence identity with AtERD2b. Error bars are standard errors. Source data are provided as a Source Data file. b CLSM analysis of two separate Arabidopsis thaliana ERD2b fluorescent fusions (YFP-TM-AtERD2 and AtERD2-YFP, shown in green), each co-expressed with the ER marker RFP-KDEL (shown in red) in HeLa cells. The endogenous cis-Golgi marker GM130 was detected via immunocytochemistry (shown in light blue) and the nucleoplasm is stained with DAPI (dark blue). Notice that in contrast to the C-terminal AtERD2-YFP fusion, YFP-TM-AtERD2 is not detected in the ER and shows the best co-localisation with GM130. The size marker bar is 10 microns. c CLSM analysis at higher magnification to compare YFP-TM-AtERD2 with two different Golgi markers. Notice that the trans-Golgi marker TGN46 is clearly distinct from the ERD2-fusion and GM130. Size marker 10 microns. d As in (b), but fluorescent fusions contain human ERD2 (HsERD2). Size marker 10 microns.