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. 2023 Mar 23;6:310. doi: 10.1038/s42003-023-04710-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a, b Two-dimensional t-SNE plots of microscopic single-cell profiling of co-cultured Caco-2 (a) and HepG2 (b) treated with 1 mM of FFAs or no treatment and stained with Calcein AM cellular and Hoechst 33258 nuclei markers (n = 3). The most distinguishable cellular parameters (AreaShape_EquivalentDiameter, Intensity_MaxIntensity_Calcein AM and Intensity_MaxIntensity_Hoechst 33258) are shown in the corresponding t-SNE plots, as well as the boxplots in Supplementary Fig. S11. The p-values are presented in Supplementary Tables S11,S12.