Figure 10.

TGFβR1 signaling is involved in PKCα-induced p21^Cip1and p27^Kip1upregulation. IEC-18 cells were pretreated with 50 nM Repsox or 5 μM GW788388 prior to treatment with vehicle (C) or 100 nM PMA (P). A and B, expression of p21^Cip1 (A) and p27^Kip1 (B), as well as phosphorylation of Smad2 (B), were determined by Western blotting. Data are representative of at least three independent experiments. IEC-18, intestinal crypt-like cells; PKCα, protein kinase C α; PMA, phorbol 12-myristate 13-acetate; TGFβR1, transforming growth factor-β receptor 1.