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. 2023 Jan 31;15(5):1071–1084. doi: 10.1016/j.jcmgh.2023.01.008

Figure 3.

Figure 3

Ninj1 deficiency in KCs restrained neutrophils but not macrophage infiltration, and inhibited intrahepatic inflammation post-IR. Chimeric mice were established as described in the Materials and Methods section. WT→WT and KO→WT mice were subjected to 90 minutes of warm ischemia or a sham procedure, and liver tissues and blood specimens were collected at 24 hours after reperfusion. (A and B) Representative immunofluorescence staining of CD11b (red) and 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) (blue) in livers from each group, quantification of CD11b+ cells per high-power field. Scale bar: 100 μm. (C and D) Representative immunofluorescence staining of LY6G (red) and DAPI (blue) in livers from each group, quantification of LY6G+ cells per high-power field. Scale bar: 100 μm. (E) Quantitative RT-PCR analysis of liver inflammatory cytokines and chemokine gene induction (IL6, IL1β, TNF-α, CXCL-1, CXCL-2, CCL1, CCL2, CCL3, and CCL5). (F) Enzyme-linked immunosorbent assay of inflammatory cytokine and chemokine levels in serum. All results are representative of at least 2 independent experiments; n = 6 mice/group. ∗P < .05. All data are presented as the means ± SD. LY6G, lymphocyte antigen 6 complex locus g.