Figure 11.

Hepatic de novo lipogenesis (DNL) genes and fatty acid beta-oxidation may not be critical in Gao-binge alcohol-induced steatosis in aged mice. (A) Western blot analysis of total liver lysates from aged mice with the indicated genotype and treatment. (B) Densitometry analysis of FASN and ACCα from (A). (C) mRNA expression analysis of Fasn and Accα in liver from aged mice with the indicated genotype and treatment. (D) Measurement of serum β-Hydroxybutyrate (D), mRNA expression of Fgf21 (E), and serum FGF21 (F) in aged mice with the indicated genotype and treatment. Results are presented as mean ± standard error (n = 3–4). ∗P < .05, one-way analysis of variance with Tukey post hoc test.