Figure 13.

Alcohol promotes liver inflammation in p62 KO aged mice. (A) Representative images of immunostaining of F4/80 resident macrophages/Kupffer cells (top), CD68 circulating macrophages (middle), and MPO (bottom) staining of infiltrated neutrophils in livers from young and aged mice with the indicated genotype and treatment. (B) Quantification of F4/80, CD68, and MPO positive cells from (A); data are presented as mean ± standard error (n = 3–4). (C) Quantitative polymerase chain reaction analysis of the indicated inflammation-related mRNAs extracted from livers of aged mice with the indicated genotype and treatment. Data are presented as mean ± standard error (n = 3–4). ∗P < .05, one-way analysis of variance followed by Tukey post hoc test.