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. 2023 Feb 7;15(5):1027–1049. doi: 10.1016/j.jcmgh.2023.01.016

Figure 2.

Figure 2

Increased hepatic p62 aggregation in aged mice after Gao-binge alcohol feeding. Young (3-month-old or 3M) and aged (14-month-old or 14M) male mice were subjected to Gao-binge alcohol model. (A) Western blot analysis of total liver lysates from WT young mice. LE, long exposure; SE, short exposure. (B) Densitometry analysis of the Western blot result from (A). (C) Western blot analysis of total liver lysates from WT aged mice. (D) Densitometry analysis of the Western blot result from (C). (E) Representative images of liver immunofluorescence staining of p62 in WT young (3M) and aged mice (14M) with or without alcohol treatment. Ctrl, control; EtOH, ethanol; scale bar: 20 μm. (F) Western blot analysis of p62 in the hepatic detergent insoluble fractions from young and aged WT mice fed with control or Gao-binge alcohol. (G) Densitometry analysis of the Western blot results from (F). Results are presented as mean ± standard error (n = 4–9).