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. 2023 Feb 7;15(5):1027–1049. doi: 10.1016/j.jcmgh.2023.01.016

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© 2023 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Impaired BAT activation in aged p62 KO mice fed with Gao-binge alcohol. (A) Representative images of gross anatomy of BAT (brown adipose tissue) sections from young and aged mice with the indicated genotype and treatment. Scale bars: 1 cm. (B) Measurement of BAT weight (in grams) and BAT to body weight ratio. (C) Representative images of H&E staining of BAT sections from young and mice with the indicated genotype and treatment. Scale bar: 50 μm. (D) Quantitative polymerase chain reaction analysis of the indicated mRNAs in BAT sections from young and aged mice with the indicated genotype and treatment. Data are presented as mean ± standard error (n = 3–5). ∗P < .05, ∗∗P < .001, ^#P < .05 between young and aged group. One-way analysis of variance followed by Tukey post hoc test.